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IRAP, REMAP and ISSR fingerprinting in newly formed hexaploid tritordeum (x Tritordeum Ascherson et Graebner) and respective parental species.

With this study, we carried out the DNA fingerprinting of two newly formed hexaploid tritordeum lines (HT22 and HT27) and their respective parents, line H1 of H. chilense and line T81 of durum wheat, based on IRAPs, REMAPs and inter-simple sequence repeats (ISSRs), in order to detect potential rearrangements in tritordeum derived from polyploidization. in...

Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences.

In the present study, we analysed mitotic chromosome spreads of tritordeum, recently produced by interspecific crosses between the H. chilense line H7 and the T. turgidum line T81 using FISH experiments with repetitive DNA sequences as probes, including SSRs, to compare their hybridization patterns and probable occurrence of genomic restructuring in the newly-formed allopolyploid. in...

Potential of Start Codon Targeted (SCoT) markers for DNA fingerprinting of newly synthesized tritordeums and respective parents.

Hexaploid tritordeum (H(ch)H(ch)AABB; 2n = 42) results from the cross between Hordeum chilense (H(ch)H(ch); 2n = 14) and cultivated durum wheat (Triticum turgidum ssp. durum (AABB; 2n = 28). Morphologically, tritordeum resembles the wheat parent, showing promise for agriculture and wheat breeding. Start Codon Targeted (SCoT) polymorphism is a recently developed technique that generates gene-targeted...

Molecular characterization of the citrate transporter gene TaMATE1 and expression analysis of upstream genes involved in organic acid transport under Al stress in bread wheat (Triticum aestivum L.).

In bread wheat, besides malate, the importance of citrate efflux for Al tolerance has also been reported. For better understanding the Al tolerance mechanism in bread wheat, here, we performed both a molecular characterization of the citrate transporter gene TaMATE1 and an investigation on the upstream variations in citrate and malate transporter genes. in Physiol...

Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivum L.)

Aluminium (Al) toxicity is considered to be one of the major constraints affecting crop productivity on acid soils. Being a trait governed by multiple genes, the identification and characterization of novel transcription factors (TFs) regulating the expression of entire response networks is a very promising approach. Therefore, the aim of the present study was to...

Differential rRNA genes expression in bread wheat and its inheritance.

In order to evaluate the inheritance of the Ag-NORs number and NOR methylation patterns, we produced F1 hybrids between bread wheat cultivars with four Ag-NORs and bread wheat cultivars with six Ag-NORs (in the direct and reciprocal senses). The F2 progenies of these F1 hybrids were also evaluated for the NOR number and methylation patterns....

Genetic diversity in Old Portuguese durum wheat cultivars assessed by retrotransposon-based markers.

Retrotransposon-based markers, such as the inter-retrotransposon-amplified polymorphism (IRAP) and the retrotransposon microsatellite-amplified polymorphism (REMAP) are highly informative, multilocus, and reveal insertional polymorphisms among plant individuals. These markers have been used for evolutionary studies, genetic diversity assessment, DNA fingerprinting, genetic mapping linkage, and for the detection of genetic rearrangements induced by polyploidization. In this study, we...

Cytogenetics, morphological, yield and molecular characterization of the Portuguese bread wheat ‘Barbela’.

“Barbela” (Triticum aestivum L. em. Thell) constitutes an aggregate of Old Portuguese landraces highly genetically diverse. We characterized 30 “Barbela” populations from four regions at the cytogenetic, molecular, morphological, and yield levels. in Plant Biosystems 145(3):540-552. Leia o trabalho completo aqui.