Genomic reshuffling in advanced lines of hexaploid tritordeum.
Genomic restructuring was detected in newly synthesized tritordeum by molecular and cytogenetic tools. Genomic stability is expected for advanced tritordeum lines (HchHchAABB; 2n = 42) with multiple generations of self-fertilization. This study intends to confirm or decline this hypothesis by characterizing three advanced tritordeum lines and their parental species using cytogenetics, inter-simple sequence repeat (ISSR)...
Genomic restructuring in F1 Hordeum chilense x durum wheat hybrids and corresponding hexaploid tritordeum lines revealed by DNA fingerprinting analyses. Journal of Genetics 96:e13–e24.
in Journal of Genetics volume 96, pages13–23. Leia do trabalho completo aqui.
Effect of allelic variation at glutenin and puroindoline loci on bread-making quality: favorable combinations occur in less toxic varieties of wheat for celiac patients.
Genetically diverse wheat samples, twenty-seven Triticum aestivum L. varieties, grown in two environments (Portugal and Spain) were analyzed for their allelic composition in high-molecular-weight glutenin subunits (HMW-GS), low-molecular-weight glutenin subunits (LMW-GS) and puroindolines, as well as their protein content, hardness, sodium dodecyl sulfate-sedimentation (SDS-S), mixograph mixing time and breakdown resistance (MT and BDR, respectively) parameters,...
Detection of genomic rearrangements in allopolyploids of the tribe Triticeae.
Tese de Mestrado de A. V. A. Delgado.
Use of the synthetic Oligo-pTa535 and Oligo-pAs1 probes for identification of Hordeum chilense-origin chromosomes in hexaploid. tritordeum.
In this study, we hybridized, for the first time, the Oligo-pTa535 and Oligo-pAs1 probes on mitotic chromosomes of hexaploid tritordeum using ND-FISH. Tritordeum (HchHchAABB) is the synthetic amphiploid resultant from crosses between wild barley (HchHch) and cultivated durum wheat (AABB). in Genetic Resources and Crop Evolution 63(6):945-951. Leia o trabalho completo aqui.
IRAP, REMAP and ISSR fingerprinting in newly formed hexaploid tritordeum (x Tritordeum Ascherson et Graebner) and respective parental species.
With this study, we carried out the DNA fingerprinting of two newly formed hexaploid tritordeum lines (HT22 and HT27) and their respective parents, line H1 of H. chilense and line T81 of durum wheat, based on IRAPs, REMAPs and inter-simple sequence repeats (ISSRs), in order to detect potential rearrangements in tritordeum derived from polyploidization. in...
Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences.
In the present study, we analysed mitotic chromosome spreads of tritordeum, recently produced by interspecific crosses between the H. chilense line H7 and the T. turgidum line T81 using FISH experiments with repetitive DNA sequences as probes, including SSRs, to compare their hybridization patterns and probable occurrence of genomic restructuring in the newly-formed allopolyploid. in...
Potential of Start Codon Targeted (SCoT) markers for DNA fingerprinting of newly synthesized tritordeums and respective parents.
Hexaploid tritordeum (H(ch)H(ch)AABB; 2n = 42) results from the cross between Hordeum chilense (H(ch)H(ch); 2n = 14) and cultivated durum wheat (Triticum turgidum ssp. durum (AABB; 2n = 28). Morphologically, tritordeum resembles the wheat parent, showing promise for agriculture and wheat breeding. Start Codon Targeted (SCoT) polymorphism is a recently developed technique that generates gene-targeted...
Molecular characterization of the citrate transporter gene TaMATE1 and expression analysis of upstream genes involved in organic acid transport under Al stress in bread wheat (Triticum aestivum L.).
In bread wheat, besides malate, the importance of citrate efflux for Al tolerance has also been reported. For better understanding the Al tolerance mechanism in bread wheat, here, we performed both a molecular characterization of the citrate transporter gene TaMATE1 and an investigation on the upstream variations in citrate and malate transporter genes. in Physiol...