Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivum L.)
Aluminium (Al) toxicity is considered to be one of the major constraints affecting crop productivity on acid soils. Being a trait governed by multiple genes, the identification and characterization of novel transcription factors (TFs) regulating the expression of entire response networks is a very promising approach. Therefore, the aim of the present study was to...
Inferences about genomic reestructuring in tritordeum based on molecular and cytogenetic markers.
Tese de Mestrado de Sandra Cabo. Leia o trabalho completo aqui.
Polymorphism of the simple sequence repeat (AAC)5 in the nucleolar chromosomes of Old Portuguese wheat cultivars.
in Journal of Genetics 92(3):583-586. Leia o trabalho completo aqui.
Differential rRNA genes expression in bread wheat and its inheritance.
In order to evaluate the inheritance of the Ag-NORs number and NOR methylation patterns, we produced F1 hybrids between bread wheat cultivars with four Ag-NORs and bread wheat cultivars with six Ag-NORs (in the direct and reciprocal senses). The F2 progenies of these F1 hybrids were also evaluated for the NOR number and methylation patterns....
Genetic diversity in Old Portuguese durum wheat cultivars assessed by retrotransposon-based markers.
Retrotransposon-based markers, such as the inter-retrotransposon-amplified polymorphism (IRAP) and the retrotransposon microsatellite-amplified polymorphism (REMAP) are highly informative, multilocus, and reveal insertional polymorphisms among plant individuals. These markers have been used for evolutionary studies, genetic diversity assessment, DNA fingerprinting, genetic mapping linkage, and for the detection of genetic rearrangements induced by polyploidization. In this study, we...
Cytogenetics, morphological, yield and molecular characterization of the Portuguese bread wheat ‘Barbela’.
“Barbela” (Triticum aestivum L. em. Thell) constitutes an aggregate of Old Portuguese landraces highly genetically diverse. We characterized 30 “Barbela” populations from four regions at the cytogenetic, molecular, morphological, and yield levels. in Plant Biosystems 145(3):540-552. Leia o trabalho completo aqui.
Physical localization of NORs and ITS length variants in old Portuguese durum wheat cultivars.
In the present study, we localized and identified the NORs of 51 old Portuguese durum wheat cultivars by using sequential silver staining and fluorescence in situ hybridization performed with the pTa71 rDNA probe. in Journal of Genetics 90(1):95-101. Leia o trabalho completo aqui.
Intergenic spacer length variants in Old Portuguese bread wheat cultivars.
The intergenic spacer of the ribosomal DNA is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. This study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of Portuguese bread wheat cultivars for IGS-amplified products obtained by PCR-RFLP...
Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal.
Using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), the different alleles encoded at the 6 glutenin loci and 3 ω-gliadin loci were identified from a set of 134 hexaploid and 128 tetraploid wheat accessions mainly grown in Portugal. in Genetic Resources and Crop Evolution, 58 (7): 1051-1073. Leia o trabalho completo aqui.
Expressão de genes rRNA e variabilidade genética em trigos antigos Portugueses.
Tese de Doutoramento de Carvalho A.I.F.