Genomic reshuffling in advanced lines of hexaploid tritordeum.
Genomic restructuring was detected in newly synthesized tritordeum by molecular and cytogenetic tools. Genomic stability is expected for advanced tritordeum lines (HchHchAABB; 2n = 42) with multiple generations of self-fertilization. This study intends to confirm or decline this hypothesis by characterizing three advanced tritordeum lines and their parental species using cytogenetics, inter-simple sequence repeat (ISSR)...
Genomic restructuring in F1 Hordeum chilense x durum wheat hybrids and corresponding hexaploid tritordeum lines revealed by DNA fingerprinting analyses. Journal of Genetics 96:e13–e24.
in Journal of Genetics volume 96, pages13–23. Leia do trabalho completo aqui.
Use of the synthetic Oligo-pTa535 and Oligo-pAs1 probes for identification of Hordeum chilense-origin chromosomes in hexaploid. tritordeum.
In this study, we hybridized, for the first time, the Oligo-pTa535 and Oligo-pAs1 probes on mitotic chromosomes of hexaploid tritordeum using ND-FISH. Tritordeum (HchHchAABB) is the synthetic amphiploid resultant from crosses between wild barley (HchHch) and cultivated durum wheat (AABB). in Genetic Resources and Crop Evolution 63(6):945-951. Leia o trabalho completo aqui.
IRAP, REMAP and ISSR fingerprinting in newly formed hexaploid tritordeum (x Tritordeum Ascherson et Graebner) and respective parental species.
With this study, we carried out the DNA fingerprinting of two newly formed hexaploid tritordeum lines (HT22 and HT27) and their respective parents, line H1 of H. chilense and line T81 of durum wheat, based on IRAPs, REMAPs and inter-simple sequence repeats (ISSRs), in order to detect potential rearrangements in tritordeum derived from polyploidization. in...
Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences.
In the present study, we analysed mitotic chromosome spreads of tritordeum, recently produced by interspecific crosses between the H. chilense line H7 and the T. turgidum line T81 using FISH experiments with repetitive DNA sequences as probes, including SSRs, to compare their hybridization patterns and probable occurrence of genomic restructuring in the newly-formed allopolyploid. in...
Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivum L.)
Aluminium (Al) toxicity is considered to be one of the major constraints affecting crop productivity on acid soils. Being a trait governed by multiple genes, the identification and characterization of novel transcription factors (TFs) regulating the expression of entire response networks is a very promising approach. Therefore, the aim of the present study was to...